Sample Sidebar Module

This is a sample module published to the sidebar_top position, using the -sidebar module class suffix. There is also a sidebar_bottom position below the menu.

Sample Sidebar Module

This is a sample module published to the sidebar_bottom position, using the -sidebar module class suffix. There is also a sidebar_top position below the search.
قسم علوم الحياة

Mokhtar J.kadhim *, Bahaa A. Lafta

Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq.

Abstract:

Two hundred and ten specimens include urine, blood and ear swab were collected from different hospitals in Baghdad city; 85 (40%) isolates were diagnosed as Proteus spp. with (82%), (11.7%) and (5.8%) in urine, blood and ear swab samples respectively. PCR technique was shown 30(35.3%) isolates positive for Urease C gene that specific for Proteus vulgaris. The ability for chondritinase production was investigated, 24(80%) isolates were able to chondritinase production and the isolate (p17) has higher enzyme activity at (175.2U/ml). The Chondroitinase was purified by short steps included precipitate with 60% ammonium sulfate, dialysis and gel filtration by Sephadex G-150. The enzymatic activity was peaked to (270U/ml) and specific activity (692.3U/mg). The enzymatic activity for purified enzyme was increased significantly (P<0.01) to (563.7U/ml) after treatment with 1M of tyrosine but aspartic acid and glutamic acid were show inhibitory effect on enzyme activity decreased (P<0.01) to (130.U/ml),(147.3U/ml) respectively. The enzymatic activity was increased significantly (P<0.01) to (633.6U/ml) and (610.3U/ml) when treated with 50% of vitamin k and  piroxicam respectively. The aim of this study is to investigate effect of amino acids, vitamins and some nonsteroidal anti-inflammatory drugs (NSAIDs) with different concentrations on enzyme activity.

Keywords: Urease C, Proteus vulgaris, Chondroitinase, Tyrosine,Vitamin k, Feldene