Sample Sidebar Module

This is a sample module published to the sidebar_top position, using the -sidebar module class suffix. There is also a sidebar_bottom position below the menu.

Sample Sidebar Module

This is a sample module published to the sidebar_bottom position, using the -sidebar module class suffix. There is also a sidebar_top position below the search.
قسم التقنيات الاحيائية

Laith  A.Yaaqoob  and Hasan  F.Al-Azzawie*

Dept.of biotechnology/College of Science/Baghdad University /Iraq

Email : hazzawie2@yahoo.com  


Abstract

 A developed, a rapid, ecofriendly and convenient green method for the synthesis of stable gold nanoparticles (AuNPs) of average diameter of 35 ± 5.0 nm and zinc oxide nanoparticals of average diameter of 40 ± 5.0 nm using the aqueous solution of Olea europeae leaves extract as reducing and capping agent used to increase the stability of the nanoparticles which characterized by UV-Vis, SEM, AFM, XRD. To test the ability of  either AuNPs or ZnO NPs to ameliorate antihyperglycemic and the oxidative stress status resulted in experimental diabetic rats induced by alloxan, One hundred and thirty male albino rats with were used in experimental design. Ten of them were served as control group G1,and anther ten male animals used as healthy control treated NPs as G2  ,while  one hundred and ten  rats were injected with alloxan at the single intraperitoneal dose of 150 mg/kg. Then, subdivided into, diabetic without any treatment G3, diabetic rats + ZnO NPs received single daily dose of three different doses(2.5,5 and 10 mg/kg b.w a G4,G5 and G6).Diabetic rats with  insulin; received a single daily subcutaneous dose of insulin 2U/kg b.w as G7 group. Diabetic rats + AuNPs received single daily dose of three different doses(2.5,5 and 10 mg/kg b.w a G8,G9 and G10) .Diabetic rats + AuNPs  + ZnO NPs received single daily dose of three different doses(2.5,5 and 10 mg/kg b.w as G11,G12 and G13).At the end of experimental time(8 weeks) the blood glucose, serum insulin,glycoslated HbA1c, MDA, GSH and serum activities of SOD, GPx and Cat were determined, in addition levels of Vit C and E and lipid profile as well histological study to liver and pancreas tissues were investigated. Results showed a significant alteration in all biochemical studied in animals treated either with ZnO NPs or  AuNPs, compared with diabetic or diabetic + insulin group and their control group. The profound control of  Oral intake of Mixture of ZnO NPs and AuNPs over the anti-oxidant enzymes in diabetic rats to normal, by inhibition of lipid peroxidation and reactive oxygen species generation during hyperglycemia evidence their antioxidant effect during diabetes. The  administration of Mixture ZnO NPs and AuNPs  at 10 mg/kg b.w exhibited an insistent control over the blood glucose level, lipids and serum biochemical profiles in diabetic rats near to the control group provokes their effective role in controlling and increasing the organ functions for better utilization of blood glucose. Histopathological studies revealed the non-toxic and protective effect of the mixture of these NPs over the vital organs and can be used to ameliorate the hyperglycemia and oxidative stress status.